YAP1 / YML007W Overview
- Standard Name
- YAP1 1
- Systematic Name
- YML007W
- SGD ID
- SGD:S000004466
- Aliases
- PAR1 35 , SNQ3 32 , PDR4 22
- Feature Type
- ORF , Verified
- Description
- Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication 2 3 4 5 6 7 8
- Name Description
- Yeast AP-1 3
- Paralog
- CAD1 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- YAP1 is located on the left arm of chromosome XIII near the centromere between MIN4 mitochondrial protein and GIS4 CAAX box protein; coding sequence is 1953 nucleotides long with a uORF at -84..-64 and 14 SNPs, 5 of which cause amino acid polymorphisms; YAP1 has paralog CAD1 from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Yap1p is 650 amino acids long, short-lived, low in abundance; contains basic-leucine zipper domain; ubiquitinylated on 3 lysines, phosphorylated on 35 residues; relative distribution to nucleus increases upon DNA replication stress
- Length (a.a.)
- 650
- Mol. Weight (Da)
- 72502.6
- Isoelectric Point
- 4.56
- Median Abundance (molecules/cell)
- 2111 +/- 977
- Half-life (hr)
- 3.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all YAP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding transcription factor involved in the regulation of transcription in response to various stresses and cellular insults; shuttles between nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor activity (IDA)
Biological Process
- Manually Curated
- involved in cellular response to oxidative stress (IDA)
- involved in positive regulation of transcription by RNA polymerase II (HMP)
- involved in regulation of endoplasmic reticulum unfolded protein response (IMP)
- involved in regulation of transcription by RNA polymerase II (IDA)
- involved in response to arsenic-containing substance (HMP)
- involved in response to heat (IMP)
- involved in response to singlet oxygen (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant show increased mutation frequency, decreased lifespan, decreased ethanol tolerance, decreased resistance to dessication, alkaline pH, resveratrol, oxidizing and reducing agents, carcinogens, antifungals, alkylating agents, metals, DNA metabolism inhibitors, protein and sterol synthesis inhibitors, and TOR inhibitor rapamycin; overexpression increases filamentous growth in Sigma1278b, interferes with cell cycle progression in S288C, and confers resistance to many of the aforementioned chemicals
Classical Genetics
- overexpression
- null
- alkaline pH resistance: decreased
- cell cycle progression in S phase: decreased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- desiccation resistance: decreased
- hydrostatic pressure resistance: decreased
- metal resistance: decreased
- metal resistance: increased
- mitotic recombination: increased
- mutation frequency: increased
- oxidative stress resistance: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: normal
- RNA accumulation: decreased
- stress resistance: decreased
- survival rate in stationary phase: increased
- toxin resistance: decreased
- transposable element transposition: increased
- viability: decreased
- viable
- gain of function
- reduction of function
- null
- competitive fitness: decreased
- competitive fitness: increased
- metal resistance: decreased
- metal resistance: increased
- mutation frequency: increased
- oxidative stress resistance: decreased
- redox state: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation efficiency: decreased
- stress resistance: decreased
- toxin resistance: increased
- UV resistance: decreased
- viable
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Yap1p interacts physically with proteins involved in transcription, YAP1 interacts genetically with genes involved in transcription; the yap1 null mutant is viable; the null mutant of paralog cad1 is viable; the yap1 cad1 double mutant displays a synthetic growth defect.
413 total interactions for 273 unique genes
Physical Interactions
- Affinity Capture-MS: 69
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 10
- Biochemical Activity: 5
- Co-localization: 1
- Co-purification: 1
- PCA: 2
- Reconstituted Complex: 10
- Two-hybrid: 32
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 2
- Dosage Rescue: 25
- Negative Genetic: 159
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 4
- Positive Genetic: 10
- Synthetic Growth Defect: 40
- Synthetic Lethality: 3
- Synthetic Rescue: 16
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- YAP1 encodes an AP-1 type transcription factor which contains a basic leucine zipper domain (bZIP), and binds to a consensus Yap1p recognition element (YRE; 5'-TT/GAC/GTC/AA-3'). Yap1p is a key player in the cellular response to oxidative stress and xenobiotic insults, including drugs and heavy metals. The leader sequence of the YAP1 mRNA contains an upstream open reading frame (uORF), that allows leaky ribosomal scanning and reinitiation to the main ORF. Activation of Yap1p occurs in response to oxidants, particularly hydrogen peroxide. The activity of Yap1p is mainly regulated at the level of its export from the nucleus. Under normal conditions Yap1p is freely distributed between cytoplasm and nucleus, but mostly it is localized in the cytosol due to its active export from the nucleus by Crm1p, which binds a nuclear export signal (NES) in the C-terminal cysteine-rich domain (CRD) of Yap1p. Yap1p is activated by glutathione (GSH) peroxidase Hyr1p, which induces a conformational change and masks the NES contained in the C-terminal CRD, thereby inhibiting Yap1p-Crm1p bond formation. Once back in the nucleus, Yap1p initiates the transcription of specific genes by binding to the YREs contained in their promoter sequences. Yap1p promotes the transcription of several genes encoding antioxidants or antioxidant-associated molecules, such as those involved in the thioredoxin (TRX) and GSH systems, including AHP1, TRR1, TRX2, TSA1, GPX2, GSH1, GSH2, GLR1, SOD1, and SOD2. Yap1p can also be activated in response to diamide, and regulates the response to cadmium by upregulating transcription of transporter YCF1. Other target genes encoding transporters include ATR1 and FLR1.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-06-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.