cdc28-1N is a conditional allele that has normal G1 (Start) function but confers a mitotic defect. We have isolated seven genes that in high dosage suppress the growth defect of cdc28-1N cells but not of Start-defective cdc28-4 cells. Three of these (CLB1, CLB2, and CLB4) encode proteins strongly homologous to G2-specific B-type cyclins. Another gene, CLB3, was cloned using PCR, CLB1 and CLB2 encode a pair of closely related proteins; CLB3 and CLB4 encode a second pair. Neither CLB1 nor CLB2 is essential; however, disruption of both is lethal and causes a mitotic defect. Furthermore, the double mutant cdc28-1N clb2::LEU2 is nonviable, whereas cdc28-4 clb2::LEU2 is viable, suggesting that the cdc28-1N protein may be defective in its interaction with B-type cyclins. Our results are consistent with CDC28 function being required in both G1 and mitosis. Its mitotic role, we believe, involves interaction with a family of at least four G2-specific cyclins.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| CLB2-CDC28 kinase complex | involved in | G2/M transition of mitotic cell cycle | IMP | ComplexPortal | 2018-04-27 | |
| CLB1-CDC28 kinase complex | involved in | G2/M transition of mitotic cell cycle | BSR | ComplexPortal | 2016-03-31 | |
| CLB2 | enables | cyclin-dependent protein serine/threonine kinase regulator activity | has input CDC28 | IMP | SGD | 2023-02-24 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| CLB2 | cell cycle progression in M phase: delayed | classical genetics | null Allele: clb2-Δ | W303 | Details: higher proportion of cells with fully replicated DNA | |
| CDC28 | cell cycle progression through the G2/M phase transition: arrested | classical genetics | conditional Allele: cdc28-1N P250L | W303 | Temperature: elevated temperature, 36 °C | |
| CLB2 | cell shape: abnormal | classical genetics | null Allele: clb2-Δ | W303 | Details: elongated cell shape | |
| CLB2 | cell size: increased | classical genetics | null Allele: clb2-Δ | W303 | ||
| CDC28 | cellular morphology: abnormal | classical genetics | conditional Allele: cdc28-1N P250L | W303 | Temperature: elevated temperature, 36 °C Details: abnormally large elongated morphology | |
| CDC28 | protein/peptide distribution: abnormal Reporter: Swi5p | classical genetics | overexpression Allele: cdc28-1N P250L | W303 | Temperature: elevated temperature, 36 °C Details: Swi5p is concentrated in the cytoplasm where it resides during G2 and M phases but not in the nucleus where it resides during the G1 phase | |
| CDC28 | protein/peptide distribution: abnormal Reporter: Swi5p | classical genetics | overexpression Allele: cdc28-4 H128Y | W303 | Temperature: elevated temperature, 36 °C Details: Swi5p is concentrated in the nucleus where it resides in G1 but not in the cytoplasm where it resides during the G2/M phase transition | |
| CLB2 | vegetative growth: decreased rate | classical genetics | null Allele: clb2-Δ | W303 | ||
| CLB1 | viable | classical genetics | null Allele: clb1-Δ | W303 |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.