The galactose metabolism positive regulatory gene from Kluyveromyces lactis, LAC9, has been isolated through its ability to activate expression of galactose metabolism enzyme genes in Saccharomyces cerevisiae. The LAC9 gene also activates expression of the S. cerevisiae alpha-galactosidase (MEL1) and K. lactis beta-galactosidase (LAC4) genes in S. cerevisiae. Although LAC9-activated gene expression in K. lactis is not glucose repressed, activation of MEL1 gene expression by LAC9 in S. cerevisiae is. The LAC9 gene is expressed at an extremely low level as a approximately 2.9-kb mRNA, and encodes a protein of 865 amino acids. Although the LAC9 gene is functionally analogous to the S. cerevisiae GAL4 gene, the bulk of its protein sequence shows little homology to that of GAL4. Two of the three regions of homology that do exist, however, are restricted to areas of GAL4 protein already implicated in nuclear localization, DNA binding, and transcriptional activation.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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