In Saccharomyces cerevisiae, efficient expression of glycolytic and translational component genes requires two DNA binding proteins, RAP1 (which binds to UASRPG) and GCR1 (which binds to the CT box). We generated deletions in GCR1 to test the validity of several different models for GCR1 function. We report here that the C-terminal half of GCR1, which includes the domain required for DNA binding to the CT box in vitro, can be removed without affecting GCR1-dependent transcription of either the glycolytic gene ADH1 or the translational component genes TEF1 and TEF2. We have also identified an activation domain within a segment of the GCR1 protein (the N-terminal third) that is essential for in vivo function. RAP1 and GCR1 can be co-immunoprecipitated from whole cell extracts, suggesting that they form a complex in vivo. The data are most consistent with a model in which GCR1 is attracted to DNA through contact with RAP1.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| RAP1-GCR1 transcription activation complex | involved in | regulation of glycolytic process | BSR | ComplexPortal | 2017-12-18 | |
| RAP1-GCR1 transcription activation complex | enables | sequence-specific DNA binding | IDA | ComplexPortal | 2017-12-18 | |
| RAP1-GCR1 transcription activation complex | located in | nucleus | BSR | ComplexPortal | 2017-12-18 | |
| RAP1-GCR1-GCR2 transcription activation complex | involved in | regulation of glycolytic process | BSR | ComplexPortal | 2017-12-18 |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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