Purified fatty acid synthetase isolated from wild type yeast cells as well as from two different fas-mutant strains was reacted with (1-14C-)iodoacetamide. Tryptic digests of the 14C-carboxamidomethylated enzymes were fractionated on Sephadex G-50. Hereby, essentially only one radioactively labeled peptide was eluted from the column. From this it is concluded that under the experimental conditions employed only the 'peripheral' SH-group of yeast fatty acid synthetase becomes alkylated. By sodium dodecylsulfate-polyacrylamide gel electrophoresis of the 14C-carboxamidomethylated fatty acid synthetase it was shown that in all three enzyme preparations studied the inhibitor is bound to the larger one of the two fatty acid synthetase subunits. These findings indicate that the larger fatty acid synthetase subunit accomodates not only the 'central' but also the 'peripheral' SH-group of the multienzyme complex.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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