Mitochondrial ribosomal proteins (mrps) of the budding yeast, Saccharomyces cerevisiae, have been extensively characterized genetically and biochemically. However, the list of the genes encoding individual mrps is still not complete and quite a few of the mrps are only predicted from their similarity to bacterial ribosomal proteins. We have constructed a yeast strain in which one of the small subunit proteins, termed Mrp4, was tagged with S-peptide and used for affinity purification of mitochondrial ribosome. Mass spectrometric analysis of the isolated proteins detected most of the small subunit mrps which were previously identified or predicted and about half of the large subunit mrps. In addition, several proteins of unknown function were identified. To confirm their identity further, we added tags to these proteins and analyzed their localization in subcellular fractions. Thus, we have newly established Ymr158w (MrpS8), Ypl013c (MrpS16), Ymr188c (MrpS17) and Ygr165w (MrpS35) as small subunit mrps and Img1, Img2, Ydr116c (MrpL1), Ynl177c (MrpL22), Ynr022c (MrpL50) and Ypr100w (MrpL51) as large subunit mrps.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| MRPS16 | respiratory growth: absent | classical genetics | null Allele: mrps16-Δ | S288C | ethanol, glycerol | |
| MRPL51 | respiratory growth: absent | classical genetics | null Allele: mrpl51-Δ | S288C | ethanol, glycerol | |
| MRPS35 | respiratory growth: absent | classical genetics | null Allele: mrps35-Δ | S288C | ethanol, glycerol | |
| MRPS8 | respiratory growth: absent | classical genetics | null Allele: mrps8-Δ | S288C | 2% ethanol, 2% glycerol | Media: nonfermentable carbon sources |
| MRPS8 | respiratory growth: absent | classical genetics | conditional Allele: MRPS8-HSV C-terminal HSV tag | Other | 2% ethanol, 2% glycerol | Media: nonfermentable carbon sources Temperature: elevated temperature, 37 °C Details: exhibits a decreased rate of respiratory growth at 30 deg C |
| MRPL1 | respiratory growth: decreased | classical genetics | null Allele: mrpl1-Δ | Other | ethanol, glycerol |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| IMG1 | MRP4 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification | |
| IMG2 | MRP4 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification | |
| MRP21 | MRP4 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification | |
| MRP4 | MRP1 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
| MRP4 | MRP13 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
| MRP4 | MRP17 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
| MRP4 | MRP2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
| MRP4 | MRP7 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
| MRP4 | MRPL1 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
| MRP4 | MRPL15 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |