Reference: Bottomley MJ, et al. (1999) A novel NMR experiment for the sequential assignment of proline residues and proline stretches in 13C/15N-labeled proteins. J Biomol NMR 13(4):381-5

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Abstract


A new pulse sequence is described for the sequential assignment of proline residues in 13C/15N-labeled proteins by correlating C delta and C alpha chemical shifts of proline residues with the H alpha chemical shift of the preceding residue. Notably, the experiment can provide the sequential connectivities in poly-proline stretches, which cannot be determined using standard triple resonance experiments. Excellent solvent suppression is achieved by coherence selection via a heteronuclear gradient echo. The new pulse sequence has been successfully applied to the 11 kDa HRDC domain.

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Journal Article | Research Support, Non-U.S. Gov't
Authors
Bottomley MJ, Macias MJ, Liu Z, Sattler M
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