This paper describes a method for the isolation of RNA polymerase A (or I) from Saccharomyces cerevisiae which is rapid and achieves a 2,000-fold purification. The method involves mainly a batchwise adsorption of enzyme on phosphocellulose and on DEAE-cellulose and a sedimentation on glycerol gradient. The enzyme obtained can be differentiated from RNA polymerase B (or II) by a number of criteria including electrophoretic migration on polyacrylamide gel, sensitivity to alpha-amanitin, subunit structure, and optimal conditions for RNA synthesis. Polyacrylamide gel electrophoresis with sodium dodecyl sulfate reveals that yeast RNA polymerase A (or I) is made up of two large subunits in equimolar amount of 190,000 and 135,000 daltons and several smaller polypeptide chains, 1 (48,000), 1 (41,000), 2 (29,000), and 2 (16,000). The RNA synthesized on native calf thymus or yeast DNA is constituted of many short chains and of a small percentage (about 10%) of very long chains which represent the bulk of the RNA. Termination factor rho from Escherichia coli inhibits 50% the transcription of native DNA.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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