During the purification of total DNA from yeast, both nuclear and mitochondrial DNA (mtDNA) molecules are obtained. Here, we describe a simple enzymatic method using a combination of λ exonuclease and RecJ(f) to obtain pure and intact mtDNA by removing linear DNA from total DNA isolated from yeast cells. The combination of the two enzymes efficiently removed linear DNA from the total DNA of Candida (Torulopsis) glabrata, leaving the mtDNA intact. The purity and integrity of mtDNA was assayed by PCR amplification of ARG1/2/5/8, URA3 and COX1, and by RFLP analysis, respectively. This method can be used to prepare mtDNA for PCR amplification or RFLP analysis without the need for purification of mitochondria by gradient ultracentrifugation or fractional precipitation. The method was also successfully applied to the yeast species Saccharomyces cerevisiae, Candida utilis, Pichia pastoris and Yarrowia lypolytica.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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