Aims: To construct novel brewer's yeast strains with the ability to degrade beta-glucan and increase sulfite levels in beer brewing by genetic manipulation.

Methods and results: The recombinant plasmid pA15ME containing P(met10)-egl1-T(met10) expression cassette was constructed. BamHI-linearized target plasmid pA15ME was transformed into the industrial brewer's yeast strain Z0103 to replace the MET10 locus through one-step gene replacement. The recombinants Z8, Z7 and Z3 with the ability to secrete active endo-beta-1,4-glucanase I into the culture medium were isolated by Congo red dyeing. The enzymatic activities of EG I of Z8, Z7 and Z3 were 3.3, 1.5, 1.3 U l(-1), and the hydrolysing degrees of beta-glucans in wort were increased 11.9%, 8.6% and 6.9%, respectively, than that of original strain Z0103. The MET10 gene deletions were confirmed by real-time PCR, and the sulfite levels of the culture mediums inoculated with Z8, Z7 and Z3 were increased 26%, 16% and 17%, respectively, compared to that of Z0103.

Conclusions: The novel endoglucanase-producing brewer's yeast strains with inserted endoglucanase gene and deficient MET10 gene led to reduced content of barley beta-glucans, enhanced filterability and increased sulfur dioxide in fermenting wort. Thus, the cost for addition of microbial beta-glucanase enzyme and sulfite preparations in normal beer brewing processes could be reduced.

Significance and impact of the study: These results suggested that genetic engineering approach is a powerful tool to construct the novel recombinant brewer's yeast strains with different properties to reduce the cost of beer brewing and improve the flavour of a beer, and the strains obtained have potential application value in beer brewing.

• PMID: 19780951
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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Lu Y, Wang TH, Ding XL

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