An extremely low acid (ELA) pretreatment using 0.06% (w/w) sulfuric acid at 170 °C for 15 min was employed to extract non-glucan components from Saccharina japonica, a brown macroalgae. Subsequent simultaneous saccharification and fermentation (SSF) was conducted using Saccharomyces cerevisiae DK 410362 and cellulase (15 FPU/g-glucan) and ß-glucosidase (70 pNPGU/g-glucan). Deionized water was used for making fermentation suspension. After the ELA pretreatment, a glucan content of 29.10% and an enzymatic digestibility of 83.96% was obtained for pretreated S. japonica. These values are 4.2- and 2.4-fold higher, respectively, than those of obtained with untreated S. japonica. In SSF, a bioethanol concentration of 6.65 g/L was obtained, corresponding to a glucose equivalent concentration of 13.01 g/L, which indicated an SSF yield of 67.41% based on the total available glucan of the pretreated S. japonica. The remaining separated liquid hydrolysate, which contains mannitol and alginate-derived oligosaccharides can be applied to other fermentations.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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