Reference: Kim HJ, et al. (2024)
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Abstract
The evolutionarily conserved HIRA/Hir histone chaperone complex and ASF1a/Asf1 co-chaperone cooperate to deposit histone (H3/H4)2 tetramers on DNA for replication-independent chromatin assembly. The molecular architecture of the HIRA/Hir complex and its mode of histone deposition have remained unknown. Here, we report the cryo-EM structure of the S. cerevisiae Hir complex with Asf1/H3/H4 at 2.9-6.8 Å resolution. We find that the Hir complex forms an arc-shaped dimer with a Hir1/Hir2/Hir3/Hpc2 stoichiometry of 2/4/2/4. The core of the complex containing two Hir1/Hir2/Hir2 trimers and N-terminal segments of Hir3 forms a central cavity containing two copies of Hpc2, with one engaged by Asf1/H3/H4, in a suitable position to accommodate a histone (H3/H4)2 tetramer, while the C-terminal segments of Hir3 harbor nucleic acid binding activity to wrap DNA around the Hpc2-assisted histone tetramer. The structure suggests a model for how the Hir/Asf1 complex promotes the formation of histone tetramers for their subsequent deposition onto DNA.
- Reference Type
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Journal Article
- Authors
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Kim HJ,
Szurgot MR,
van Eeuwen T,
Ricketts MD,
Basnet P,
Zhang AL,
Vogt A,
Sharmin S,
Kaplan CD,
Garcia BA,
... Show all
Marmorstein R,
Murakami K
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- HHF1 | HHT1 | HIR3 | HIR1 | HPC2 | ASF1 | HHF2 | HHT2 | HIR2 | HIR complex
Gene Ontology Annotations
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| Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
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Reference |
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| Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
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Disease Ontology Term |
Disease Ontology Term ID |
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| Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
Happens During |
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Post-translational Modifications
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Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| ASF1 | HIR2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| HHF1 | ASF1 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification |
| HHF1 | HHT1 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification |
| HHF1 | HIR2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| HHF1 | HIR3 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| HHT1 | ASF1 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification |
| HHT1 | HIR2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| HHT1 | HIR3 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| HHT1 | HPC2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| HIR1 | HIR2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
Functional Complementation Annotations
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| Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |