Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
9 entries for 4 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| YPT6 | heat sensitivity: increased | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | Temperature: elevated temperature, 35 °C Details: growth defect partially rescued by 1 M sorbitol | Luo Z and Gallwitz D (2003) PMID:12401784 | |
| YPT6 | protein transport: delayed Reporter: carboxypeptidase Y | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | Temperature: elevated temperature, 37 °C Details: reduced transport kinetics of CPY at 37 deg C; delayed maturation and partial missorting of the Golgi-glycosylated (p2) form to the extracellular space at 25 deg C | Luo Z and Gallwitz D (2003) PMID:12401784 | |
| YPT6 | protein transport: delayed Reporter: carboxypeptidase Y | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | Temperature: elevated temperature, 37 °C Details: reduced transport kinetics of CPY at 37 deg C; delayed maturation and partial missorting of Golgi-glycosylated (p2) form to the extracellular space at 25 deg C | Luo Z and Gallwitz D (2003) PMID:12401784 | |
| YPT6 | protein/peptide modification: decreased Reporter: alkaline phosphatase | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | Temperature: elevated temperature, 37 °C Details: maturation of ALP is reduced and pro-ALP accumulates | Luo Z and Gallwitz D (2003) PMID:12401784 | |
| YPT6 | protein/peptide modification: decreased Reporter: carboxypeptidase Y | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | Temperature: elevated temperature, 37 °C Details: smear between p2 and p1 CPY, indicative of a severe Golgi-specific glycosylation defect | Luo Z and Gallwitz D (2003) PMID:12401784 | |
| YPT6 | protein/peptide modification: decreased Reporter: invertase | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | Temperature: elevated temperature, 37 °C Details: underglycosylated form of invertase accumulates in the cell | Luo Z and Gallwitz D (2003) PMID:12401784 | |
| YPT6 | protein/peptide modification: decreased Reporter: Sec22-alpha factor fusion | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | Temperature: elevated temperature, 37 °C Details: defect in Golgi-to-ER and/or retrograde Golgi trafficking; observe increased Sec22p cleavage which occurs in the late Golgi | Luo Z and Gallwitz D (2003) PMID:12401784 | |
| YPT6 | resistance to chemicals: decreased | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | 0.005% sodium dodecyl sulfate | Temperature: permissive temperature, 30 °C Details: indicative of a cell wall defect | Luo Z and Gallwitz D (2003) PMID:12401784 |
| YPT6 | resistance to chemicals: decreased | classical genetics | conditional Allele: ypt6-2 G139D; helical region after the nucleotide binding motif (NKXD) mutated | Other | 0.005% Calcofluor White | Temperature: permissive temperature, 30 °C Details: indicative of a cell wall defect | Luo Z and Gallwitz D (2003) PMID:12401784 |
This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | MetaboGeneCard | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome
BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap | YeastRC Two-Hybrid