Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
8 entries for 2 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| HRQ1 | chromosome/plasmid maintenance: abnormal | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | Details: increased rate of gross-chromosomal rearrangements | Bochman ML, et al. (2014) PMID:24440721 | |
| HRQ1 | chromosome/plasmid maintenance: abnormal | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | mitomycin C | Details: greatly increased rate of gross-chromosomal rearrangements | Bochman ML, et al. (2014) PMID:24440721 |
| HRQ1 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | 10 mM caffeine | Details: supersensitive relative to wt | Rogers CM, et al. (2020) PMID:33115721 |
| HRQ1 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | 25 μg/mL diepoxybutane | Details: Diepoxybutane induces DNA inter-strand crosslinks | Rogers CM, et al. (2020) PMID:32371399 |
| HRQ1 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | 50 μg/mL mitomycin C | Details: Mitomycin C induces DNA inter-strand crosslinks | Rogers CM, et al. (2020) PMID:32371399 |
| HRQ1 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | 100 μg/mL mitomycin C | Bochman ML, et al. (2014) PMID:24440721 | |
| HRQ1 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | 125 ng/mL 4-nitroquinoline N-oxide | Bochman ML, et al. (2014) PMID:24440721 | |
| HRQ1 | resistance to chemicals: decreased | classical genetics | reduction of function Allele: hrq1-KA K318A; Walker A box invariant lysine; catalytically inactive, lacking helicase activity; binds ssDNA almost as well as WT | S288C | 250 μg/mL cisplatin | Bochman ML, et al. (2014) PMID:24440721 |
This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | MetaboGeneCard | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome
BioGRID | CYC2008 | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap