Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
6 entries for 4 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| BRL1 | inviable | classical genetics | null Allele: brl1-F391E F391E; hydrophobic part of the amphipathic helix (AαH) mutated | Other | Details: unable to support growth at 23, 30 and 37 deg C | Vitale J, et al. (2022) PMID:35293775 | |
| BRL1 | nuclear morphology: abnormal | classical genetics | overexpression Allele: brl1-F391E F391E; hydrophobic part of the amphipathic helix (AαH) mutated | Other | Details: formation of petal-like structures, large cytoplasmic-directed inner and outer nuclear membrane deformations that carry nuclear pore proteins at their base | Vitale J, et al. (2022) PMID:35293775 | |
| BRL1 | protein/peptide distribution: abnormal Reporter: Nsp1p-tdTomato | classical genetics | overexpression Allele: brl1-F391E F391E; hydrophobic part of the amphipathic helix (AαH) mutated | Other | Details: loss of uniform nuclear envelope distribution of the cytoplasmic nucleoporin Nsp1p; 35% of cells display colocalization of Nsp1p with petal-like extensions of the nuclear envelope | Vitale J, et al. (2022) PMID:35293775 | |
| BRL1 | protein/peptide distribution: abnormal Reporter: Nup159-tdTomato | classical genetics | overexpression Allele: brl1-F391E F391E; hydrophobic part of the amphipathic helix (AαH) mutated | Other | Details: loss of uniform nuclear envelope distribution of the cytoplasmic nucleoporin Nup159p; 43% of cells display colocalization of Nup159p with petal-like extensions of the nuclear envelope | Vitale J, et al. (2022) PMID:35293775 | |
| BRL1 | protein/peptide distribution: abnormal Reporter: Nup42p-tdTomato | classical genetics | overexpression Allele: brl1-F391E F391E; hydrophobic part of the amphipathic helix (AαH) mutated | Other | Details: loss of uniform nuclear envelope distribution of the cytoplasmic nucleoporin Nup42p; 42% of cells display colocalization of Nup42p with petal-like extensions of the nuclear envelope | Vitale J, et al. (2022) PMID:35293775 | |
| BRL1 | vegetative growth: decreased | classical genetics | overexpression Allele: brl1-F391E F391E; hydrophobic part of the amphipathic helix (AαH) mutated | Other | Details: severe growth defect | Vitale J, et al. (2022) PMID:35293775 |
This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome
BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap