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Allele: pif1-T464A

Allele Name
pif1-T464A 1
SGD ID
SGD:S000350057
Affected Gene
PIF1
Allele Type
missense variant 1
Description
T464A; DNA binding site mutated; impaired DNA unwinding activity, reduced affinity for DNA and a reduced rate of ATP hydrolysis 2

Phenotype Annotations

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

2 entries for 2 phenotypes

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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
PIF1chromosome/plasmid maintenance: decreased
classical genetics reduction of function
Allele: pif1-T464A

T464A; DNA binding site mutated; impaired DNA unwinding activity, reduced affinity for DNA and a reduced rate of ATP hydrolysis

S288CDetails: 34-fold increase in the rate of gross-chromosomal rearrangements (GCRs) using a fluctuation test to assay for the simultaneous loss of CAN1 and URA3 markers on the left arm of chromosome V
Gao J, et al. (2024) PMID:38752483
PIF1telomere length: increased
classical genetics reduction of function
Allele: pif1-T464A

T464A; DNA binding site mutated; impaired DNA unwinding activity, reduced affinity for DNA and a reduced rate of ATP hydrolysis

S288CDetails: increased telomere length relative to wt, assayed by evaluating Y′-telomeric length using Southern blot
Gao J, et al. (2024) PMID:38752483
Showing 1 to 2 of 2 entries

Shared Alleles

This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.

References

List of references used specifically to curate the information in Overview section.

  1. Gao J, et al. (2024) Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth. Nucleic Acids Res 52(11):6543-6557 PMID: 38752483
  2. Zhou J, et al. (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science 289(5480):771-4 PMID: 10926538

Resources

Mutant Strains

Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC

Phenotype Resources

dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | MetaboGeneCard | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome

Ontology

Yeast Phenotype Ontology

Interactions Resources

BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap